PMC

Spore production of four pathogen lineages on Dianthus pavonius of infected plants in Experiment 1 that flowered in the first year. Colors and shapes indicate host-of-origin: Pink circles-Dianthus pavonius, Green squares- D. seguieri, Blue triangles-D. furcatus. Neither lineage nor host-of-origin was a significant predictor of spore production ().

In situ Salkowski staining on plant roots treated with different Klebsiella SGM 81 and control plants. Visual localisation of IAA using Salkowski reagent on roots of Dianthus caryophyllus treated with: a 10² CFU.mL⁻¹, b Control plants. The development of pinkish red colour proximal to root indicates the presence of auxin. The control plants do not show pink colour upon addition of the reagent which indicates the absence of any exogenous bacterial IAA

Comparison of the A) potential and B) realized host range of four Microbotryum lineages (L1-L4). A) Infection results from Experiment 1. Bars show the proportion of each host species infected by pathogen lineages L1-L4. Colors indicate host species: Dianthus pavonius (pink), D. seguieri (green), or D. furcatus (blue). Numbers indicate the total number that flowered and were scored. B) Field collections of the distribution of Microbotryum lineages on Dianthus species are shown for reference (data redrawn from ). Bars show the proportion of isolates from each lineage that were found on each host. Numbers of isolates in each category are indicated at the bottom. Error bars are 1 SEM.

Structure-based amino acid sequence alignment of enzymes belonging to the phylogeny clade of the PEPM/ICL superfamily whose members exhibit substrate promiscuity. Mg²⁺/Mn²⁺ coordinating residues are highlighted in red, as are several other key active site residues, except the catalytic Cys and Glu are highlighted in yellow. The α-helical and β-strand secondary structure units, as defined in the reported OAH structure, are depicted above the sequence blocks. Abbreviations uses are as follows: Cp, C. parasitica (GenBank^TM accession number GU932672); Bf, B. cinerea; Ag, A. niger; Dc, Dianthus caryophyllus (carnation, clover pink).